Direct ultramicro glucose oxidase method for determination of glucose in biologic fluids.
نویسندگان
چکیده
IAULLFR (1) first demonstrated that fl-glucose oxidase promoted the oxidation of glucose by molecular oxygen to gluconic acid. Franke and Lorenz (2) discovered that hydrogen peroxide is simultaneously produced in this reaction. Keston (3), Comes (4), and Free et at. (5) made the first practical application of the use of glucose oxidase in the estimation of glucose in urine by impregnating paper strips with glucose oxidase, horse-radish peroxidase and o-tolidine as indicator. The hydrogen peroxide formed from the oxidation of glucose by glucose oxidase is used to oxidize o-tolidine to a blue color in the presence of peroxidase.
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 6 شماره
صفحات -
تاریخ انتشار 1960